| Date | Home | Away | Result |
|---|---|---|---|
| 31/10/25 19:30 | Luton Town | Forest Green Rovers | 4-3 |
| 01/11/25 12:00 | Chelmsford City | Braintree Town | 4-1 |
| 01/11/25 15:00 | Wycombe Wanderers | Plymouth Argyle | 2-0 |
| 01/11/25 15:00 | Halifax Town | Exeter City | 0-2 |
| 01/11/25 15:00 | Boreham Wood | Crawley Town | 3-0 |
| 01/11/25 15:00 | Bolton Wanderers | Huddersfield Town | 2-1 |
| 01/11/25 15:00 | Oldham Athletic | Northampton Town | 3-1 |
| 01/11/25 15:00 | Crewe Alexandra | Doncaster Rovers | 1-2 |
| 01/11/25 15:00 | Stevenage FC | FC Chesterfield | 0-1 |
| 01/11/25 15:00 | Barnsley FC | York City | 3-2 |
| 01/11/25 15:00 | Reading FC | Carlisle United | 2-3a.e.t |
| 01/11/25 15:00 | Bromley FC | Bristol Rovers | 1-2 |
| 01/11/25 15:00 | Peterborough United | Cardiff City | 1-0 |
| 01/11/25 15:00 | AFC Wimbledon | FC Gateshead | 0-2 |
| 01/11/25 15:00 | Mansfield Town | Harrogate Town FC | 3-2 |
| 01/11/25 15:00 | Fleetwood Town | FC Barnet | 2-1 |
| 01/11/25 15:00 | FC Blackpool | Scunthorpe United | 1-0 |
| 01/11/25 15:00 | Wealdstone FC | Southend United | 1-0 |
| 01/11/25 15:00 | Rotherham United | Swindon Town | 1-2a.e.t |
| 01/11/25 15:00 | Grimsby Town | Ebbsfleet United | 3-1 |
| 01/11/25 15:00 | Tranmere Rovers | Stockport County | 1-3 |
| 01/11/25 15:00 | Wigan Athletic | Hemel Hempstead Town | 6-4a.PSO |
| 01/11/25 15:00 | Newport County | FC Gillingham | 6-5a.PSO |
| 01/11/25 15:00 | Cheltenham Town | Bradford City | 1-0 |
| 01/11/25 15:00 | Salford City | Lincoln City | 5-3a.PSO |
| 01/11/25 15:00 | Colchester United | Milton Keynes Dons FC | 2-3 |
| 01/11/25 15:00 | Weston-Super-Mare | Aldershot Town | 2-1a.e.t |
| 01/11/25 15:00 | Spennymoor Town | Barrow FC | 0-2 |
| 01/11/25 15:00 | Slough Town FC | Altrincham FC | 2-1 |
| 01/11/25 15:00 | Sutton United | Telford United | 2-1 |
| 01/11/25 15:00 | Burton Albion | St Albans City | 6-0 |
| 01/11/25 15:00 | Cambridge United | Chester FC | 3-0 |
| 01/11/25 15:00 | Buxton FC | Chatham Town | 2-1a.e.t |
| 01/11/25 15:00 | Macclesfield FC | AFC Totton | 6-3 |
| 01/11/25 17:30 | Brackley Town | Notts County | 6-5a.PSO |
| 02/11/25 12:00 | South Shields FC | Shrewsbury Town | 1-3 |
| 02/11/25 14:15 | Eastleigh FC | FC Walsall | 0-3 |
| 02/11/25 15:00 | FC Port Vale | Maldon and Tiptree FC | 5-1 |
| 02/11/25 17:15 | Gainsborough Trinity | Accrington Stanley | 1-2a.e.t |
| 03/11/25 19:30 | FC Tamworth | Leyton Orient | 0-1 |