| Date | Home | Away | Result |
|---|---|---|---|
| 29/11/24 19:45 | Harrogate Town FC | Gainsborough Trinity | --- |
| 30/11/24 11:30 | Wealdstone FC | Wycombe Wanderers | --- |
| 30/11/24 15:00 | FC Walsall | Charlton Athletic | --- |
| 30/11/24 15:00 | Salford City | Cheltenham Town | --- |
| 30/11/24 15:00 | Accrington Stanley | Swindon Town | --- |
| 30/11/24 15:00 | Barnsley FC | Bristol Rovers | --- |
| 30/11/24 15:00 | Leyton Orient | Oldham Athletic | --- |
| 30/11/24 15:00 | Exeter City | FC Chesterfield | --- |
| 30/11/24 15:00 | FC Morecambe | Bradford City | --- |
| 30/11/24 15:00 | Stockport County | Brackley Town | --- |
| 30/11/24 15:00 | Peterborough United | Notts County | --- |
| 30/11/24 15:00 | Stevenage FC | Mansfield Town | --- |
| 30/11/24 15:00 | Cambridge United | Wigan Athletic | --- |
| 30/11/24 15:00 | Crawley Town | Lincoln City | --- |
| 30/11/24 19:15 | AFC Wimbledon | Dagenham and Redbridge | --- |
| 01/12/24 12:00 | Kettering Town FC | Doncaster Rovers | --- |
| 01/12/24 13:00 | FC Blackpool | Birmingham City | --- |
| 01/12/24 14:00 | Burton Albion | FC Tamworth | --- |
| 01/12/24 14:00 | Reading FC | Harborough Town FC | --- |
| 01/12/24 15:15 | Solihull Moors FC | Bromley FC | --- |